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Characterising Multivalent Complex Formation Using Controlled Complex Lifetime in Fida 1
Emil Georg Palmkvist Vangsgaard Stender, PhD, Kritika Sahni Ray, Dr. rer. nat.
This Fidabio poster demonstrates how Flow Induced Dispersion Analysis (FIDA), on a Fida 1 instrument, was used to characterise multivalent complex formation between bovine β-lactoglobulin (BLG) and polyclonal anti-BLG IgG, using a capillary-mixing (Capmix) method to control complex lifetime. By varying the in-capillary mixing time, FIDA decoupled the primary binding interaction from downstream agglutination and quantified binding affinity (KD), complex size and oligomer formation. It is an example of using FIDA to characterise multivalent protein–protein interactions and distinguish true binding from aggregation-driven artefacts.
