Targeted Protein Degradation

In-depth characterisation – within minutes, without a doubt.
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What does FIDA bring to TPD research?


Effective degrader design

  • Direct measurement of degrader constructs
  • Ternary complex validation by direct measurement of changes in size
  • Hit-characterisation as follow-up to high throughput screens
  • Affinities and functionality of the degraders characterised in 4 minutes
  • Absolute size measurement of the complexes eliminates second-guessing on Kds of all binding partners, incl. cooperativity and sample integrity

Directly in cell lysate

  • Measure interactions with GFP/YFP tagged proteins
  • FIDA users save time by not having to purify their proteins

Low sample consumption

  • 〜10x less sample consumed than in most SPR technologies
  • Work directly in unpurified samples
  • Down to 4 μL analyte, 40 nL indicator
  • Recover your sample material

Affinity measurement readout even for weak interactions

  • The most sensitive affinity measurement on the market
  • Affinity from high pM to mM Kds

Direct and in-vitro detection of ubiquitination

  • Save time and energy otherwise spent on SDS-page and western blot

Built-in quality control in every data point to increase data reliability

  • 8 parameters: Absolute size, Polydispersity index, Sample loss, PDB correlator, Aggregation, Viscosity, Stickiness, Labelling quality


Determine binding interactions under physiological conditions
Know Kds of all binding partners
Know the direct, absolute size
Clear and easy to interpret data dashboard
Speed to data
Troubleshoot efficiently
On-spot analysis kept in-lab


Directly in cell lysate, independent of buffer conditions
Measure cooperativity, even for weak interactions
Sample integrity control
Low sample usage - 4μL analyte, 40 nL indicator
Dedicated TPD software module
Calibration free assay


Accurate determination of dilute phase concentration
Relative droplet size distributions
Kinetics of droplet formation and maturation into amyloid fibrils
Determination of binding affinity between the polypeptide undergoing LLPS and LLPS-modulating compounds

Full quantitative ternary complex characterisation

FIDA provides our users with a full, quantitative characterisation of the ternary complex and its subunits, including Kds of all binding partners, cooperativity factor and sample integrity control.

All under any buffer condition and in complex media, including cell lysate.

  1. Quantitative determination of ternary complex formation
  2. Determination of all construct components bindings, including cooperativity
  3. Determination of the bound fraction of the protein of interest to the ternary complex

Save months of work – FIDA users meet their goals ahead of their competitors

  1. FIDA’s quality control delivers users clear information about the quality of their samples – allowing for troubleshooting in minutes, not days, and saving our users from many frustrations.
  2. FIDA is already used at the expression and pre-purification stage in order to indicate if degraders are functional. This helps to save our users from expensive late stage attritions.
  3. FIDA assays are ready within a few hours, even in the case of new users.

Explore improved workflow opportunities

Thanks to in-depth characterisation, FIDA narrows down the number of leads identified through high-throughput screening

  1. FIDA is employed in diverse workflow stages –
    before or after purification.
  2. It is used for QC, lead characterisation and dedicated parameter determination.
  3. FIDA workflows are shorter thanks to running assays on site – with FIDA there is no need to send out samples to specialised SPR departments

Stay independent

  1. After a short training by our dedicated FIDA TPD team, users run assays on spot and in their own development lab.
  2. With FIDA, there is no more need to send out the samples to external analysis departments.

Improving drug development productivity

  1. In-depth and rapid quality control delivered by FIDA facilitates and speeds up the evaluation
    process in the hit-to-lead stage of drug
  2. FIDA users increase their efficiency of screening hits, and reduce iterations thanks to FIDA’s first principle nature, the ability to run under native conditions, and the comprehensive built-in QC.

Targeted Protein Degradation


Targeted Protein Degradation

tartgeted protein degradation tdp workflow

Targeting common challenges in research on TPD

TPD Challenge
Identify efficient target protein degradation candidates
Screen libraries of degraders – Thanks to FIDA’s capacity to monitor even minute changes in the size of the target protein as a response to different compounds, FIDA can be used for assessment of degraders’ ability to induce degradation of targeted protein.
Occurrence of ubiquitination
Compared to e.g. SDS-PAGE and western blots, FIDA enables real-time monitoring of the ubiquitination reaction in 15–30 mins
Evaluating selectivity
FIDA can be used to analyse whether your candidates actually measure changes in the size of any protein, including the non-target ones. This can make it a tool to evaluate off-target effects of degraders.
Effective degrader design
The dedicated FIDA ternary complex software module enables users to determine the relevant affinities under diverse conditions. This information facilitates and speeds up the selection of degraders with optimal properties.
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Become a user

Your laboratory instruments should serve you, not the other way around. We’re happy to help you.