Lipid Nano-particles

Binding analytics - affinity measurements
I'm Interested
lipid nanoparticles
lipid nanoparticle

How does FIDA accelerate LNP development?


Study LNP binding interactions in complex matrices

  • As an in-solution, non-immobilization technology FIDA enables characterization of interactions on surface of large particles.
  • Study LNPs directly in serum, thus obtain clinically relevant data.
  • No buffer constraints – FIDA users have wide research design flexibility.

Detect even weak binders

  • Study interactions on large particles
  • Measure interactions over pM to mM – with a single technology

Screen LNPs for specific tissue targets

  • FIDA can be used as a pre-screening, in vitro technology to decrease the need for costly and lengthy animal studies.
  • In-vitro, in-solution, serum-based screens for studying interactions with organ-specific proteins can yield data for reducing the size of animal-studies.

Characterisation of tissue specific proteins

  • FIDA can be used for in-depth characterization of proteins identified in proteomic screens.
  • Precise and absolute size measurement ensures high confidence in results accuracy.

Quantitative comparisons of LNPs

  • Comparative (quantitative) screening of LNPs - possible thanks to FIDA’s precision - it can detect size changes down to an angstrom.
  • Outperforms other technologies in terms of sizing accuracy and interaction measurement sensitivity

Technological flexibility of an open architecture platform

  • Multiple detectors allow for label or label-free assays
  • Temperature control for analysis and sample storage
  • Minimal buffer constraints
  • Embedded viscosity measurement
  • Robust, user-friendly design

Versatile for usage in LNP research, development and manufacturing

  • Open-platform and straightforward system facilitates processes
  • Absolute measurements ensure replicability


Delivers clinically relevant findings thanks to in-solution serum or plasma based studies.
Delivers precise size measurements (down to 5% in size change)
Provides versatility thanks to an open-platform format with multiple detectors
Allows to study even weak target-specific bindings
Lower costs thanks to the ability to pre-screen samples
In-depth characterisation opportunities thanks to absolute measurements


No buffer constraints
In-solution, non-immobilization
Multiple Detectors: UV (label free), 480, 535, 640
User-friendly software


Accurate determination of dilute phase concentration
Relative droplet size distributions
Kinetics of droplet formation and maturation into amyloid fibrils
Determination of binding affinity between the polypeptide undergoing LLPS and LLPS-modulating compounds

One technology throughout the workflow:

Scaling for production

Targeting common challenges in research on lipid nanoparticles

Size and Stability Control
FlDA allows its users to determine the absolute size (Rh) of lipid nanoparticles under dynamic flow conditions. It also allows to quantify aggregates, that may affect the overall quality and performance of the LNPs under development.
Biocompatibility and Toxicity
FIDA users work in flexible buffer conditions (serum, plasma or cell lysate), simulating different physiological environments.
Scalability and Manufacturing
FIDA is employed as a quality control tool during the manufacturing process to ensure consistency and stability of the product.

This methodology is very sensitive and useful for assessing binding in a quantitative manner using small amounts of auto-fluorescent proteins. Using this method, we can observe clear changes in apparent hydrodynamic radius (Rh) and in fluorescence signal of EYA1 as a function of increasing concentrations of peptides or of small molecules

Dana Farber Institute

''Fida  Instrument provides a ''ruler'' to measure the length of fibrils''

Stefan Rüdiger Ph.D.
Professor of Protein Chemistry of Disease; Head of Department of Chemistry

''The FIDA is very effective for screening small molecules for solubility and interactions with protein targets. A major advantage has been its ability to clearly show drug binding to difficult-to-work-with targets such as intrinsically disordered proteins and amyloids''

Dr. Alexander Taylor
Research Fellow in Biochemistry/Biophysics

I would say that this instrument is quite useful for core facilities at KI or for Swedish research community in general:

  1. it was possible to measure unlabeled membrane proteins and soluble proteins at low concentrations in small volumes very fast (~6 minutes per measurement).
  2. I also like the temperature control and the autosampler which allowed us to run a lot of samples overnight.
  3. I would say that there is a lot of potential to perform other assays as well, such as the Kd determinations etc. (For this, I use ITC which is quite time-consuming and requires a lot of protein).
  4. In addition, the software and user interface for sample runs and data analysis is quite straightforward and intuitive to use.
Postdoctoral Researcher

"What we value about FIDA is the amount of information we can get from a tiny amount of protein in a single QC run. FIDA technology enables us to understand our proteins better - particularly when they behave in unexpected ways. We chose FIDA because it gives us that vital first result quickly - we don't waste our time optimising an assay that isn't going to work."

Duncan Smith
Lead Scientist

"I am convinced. I have never before been able to detect the ternary construct formation of my construct directly in cell lysate – it took less than two hours."

Thomas Smith
Associate Director, Chemical Biology & Therapeutics

"The Fida 1’s unique capability for measuring binding Kd from weak mM level to strong pM level really enhances our bioanalytical capabilities."

Sherry Zhu
Senior Scientist

"FIDA is an in-solution technique, and thus an ideal binding assay for structurally diverse bsAbs because the analysis does not rely on potentially obstructive surface immobilization and hence it is able to perform characterizations that are unbiased by bsAb format and spatial orientation of the binding domains."

Andreas V. Madsen
PhD Student

"After thorough comparisons, it was clear that the Fida 1, amongst others, presents unique opportunities in analysis of membrane proteins, which is essential to us."

Svend Kjæer
STP Deputy

''The Fida 1 system offers a new approach to detergent screening that has significant advantages allowing essential data to be obtained faster using less material (…) this work presents a new approach to characterize membrane proteins that allows users to reducing costs and time as well as to analyze protein expressed at low levels in a short time thus overcoming any stability issues.''

Isabel Moraes
Principal Research Scientist

"I like the ease of use and the straightforward data analysis and, that experiments can be performed in any type of buffers. We get data, where no other biophysical methods worked before."

Cathrine Birck
Head of Integrated Structural Biology Platform

"The method is easy to setup in any standard laboratory and can be adopted for a high throughput (HTTP) screening, which enabled mechanistic studies of RNA nuclease interactions, as well as efficient guide RNA lead discovery (…) Fida 1 offers straightforward assay development, walk away automation, absolute measurement and ultra low sample consumption."

Denny Truong
Principal Research Associate

"FIDA provides in-depth assessment of activity combined with local and global protein structural changes by measuring the overall hydrodynamic radius of the protein with minimal sample consumption. In addition, it is possible to measure the affinity constant for the analyzed interaction."

Dr. Melanie Hug
Research Associate

"The beauty of Fida 1 is that it allows us to conduct a lot of biophysical measurements in one system using a small amount of reagent and a rapid read-out time which is important for our projects and to our customers. (…) Providing a high-quality, protein characterisation QC package is certainly extending and future proofing our capabilities in biophysical characterisation of the proteins we produce."

Mark Abbott
Managing Director

"The Fida 1 has allowed us to assess the performance of MIPs with a much higher throughput than was previously possible on other platforms. MIPs can be developed in as little as 8 weeks, which is a significant improvement on antibodies, and now they can be fully characterized at an accelerated pace too."

Alan Thomson

"Fida 1 can be used for full characterization of ternary complex formation for targeted protein degradation. The Fida 1 platform only consumes 39 nL of sample for one measurement and thus allows elaborate condition screening using very small amounts of sample material."

Roman Agafonov
Associate Director of Biochemistry, Biophysics & Structural Biology

"The Fida1 is a very user-friendly system. With the Fida software we can quickly design new methods, set up experiments, and analyze data. By far this is a great instrument and technology that has allowed us to run binding assays for LNP in solution and with flexibility to run in any complex media which we were unable to do using traditional binding methods that required ligand immobilization. The FidaBio customer service is amazing, they are very knowledgeable and responsive whenever I run into issues and need assistance. I highly recommend this instrument for LNP or any other nanoparticle characterization. The system and technology are versatile with applications beyond nanoparticles and is a must in the biophysical tool kit."

Biophysical Analytical Department
Senior Scientist, Analytical Development

"Eventually, with FIDA, we managed to characterise our LNPs. And it was done in less than 2 days. We had for a long time been struggling with SPR."

Chemical Biology and Therapeutics
Associate Director

"We had been looking for ways of quantifying our exosomes, and tested many platform, without success. In less than 2 days FIDA had developed an assay and shown trustworthy quantifications directly in fermentation broth"

Rane Harrison
Director, Analytical Development

Become a user

Your laboratory instruments should serve you, not the other way around. We’re happy to help you.