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Interactions between gRNA and CRISPR Nuclease preface in vitro cleavage activities
Linh B. Truong, Qiyuan Yang, Tanya Jain, Grace Mekiliuwa, Jeffrey Haswell, Sejuti Sengupta1, Carl Co, John E. Murphy
This poster from Arbor Biotechnologies demonstrates how Flow Induced Dispersion Analysis (FIDA) was used to measure the binding affinity between guide RNAs (gRNAs) and CRISPR-Cas nuclease by tracking the change in RNA size as nuclease concentration increased. FIDA showed that guide-RNA modifications tune nuclease binding strength, which in turn tracked with downstream genome-editing efficiency in cells. It is an example of using FIDA to characterise protein–nucleic-acid binding and guide the design of CRISPR guide RNAs.
