50x faster screening with FIDA
How did Tozaro boost their screening throughputfrom 6 to up to 288 samples daily, reducing monthsof work to just days?
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Tozaro (Previously MIP Discovery / Diagnostics) ingeniously reimagined its affinity reagentscreening workflow using FIDA, transforming interaction analysis from slow and laborintensive to fast and precise. The benefit? They managed to achieve greater efficiency, scalability, and decision-making power in molecular interaction analysis.
Problem
Traditional techniques such as Surface Plasmon Resonance (SPR) createdthroughput bottlenecks, limiting the ability to evaluate large polymer libraries quickly andcost-effectively.
Solution
FIDA-Driven Workflow (presented below). The introduction of a primary screen performed by FIDA allowed for a rapid elimination of non-binders.
1. Primary Screen →
Rapid elimination of non-binders using afluorescently labeled indicator. Throughput: up to 3× 96-well plates/day (288 samples).
2. Confirmatory Screen →
Quadruplicate measurements to validate interactions.
3. Kd Determination →
Quantitative ranking via 8-point titration in 1.5–2 hours per sample.
4. Reverse Titration →
Confirming specificity & binding stoichiometry.
After these steps, only selected candidates are progressed to SPR to assess surfaceimmobilization effects, eliminating the need for SPR in primary screening. By their innovativeadaptation of FIDA, Tozaro replaced SPR for most polymer interaction screening analyses,streamlining research and accelerating discovery.
The benefits:
By replacing SPR with FIDA for polymer interaction analysis, Tozaro streamlined research, reduced time-to-decision, and dramatically increased efficiency in polymer screening workflows.
SPR: 16+ days → ~6 polymers/day
FIDA: 30min - 3 hours → up to 288 polymers/day
Would you like to increase your efficiency as well?
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If we sparked your curiosity, go ahead, book a discovery call to explore if FIDA could be something just right for you.