How is FIDA used in computational protein design?
Direct in-lysate screening of synthetic proteins
- Analyze binders directly in unpurified or heat-treated lysate (or complex media).
- No purification, or immobilization required.
- Maintains native interaction conditions.
Quantitative affinity & size measurement
- One platform delivers: binding screening, Kd, and aggregation measurements.
- No false-positives, thanks to first-principle measurements.
- Robust: Absolute, quantitative data.
High sensitivity, low
sample use
- Highly sensitive: Detect small differences between similar de novo mini-binders.
- Down to 40 nL volumes.
- 96-well plate workflows.
Workflow-ready for binder development
- Screen, rank, and characterize your de novo binders in one workflow.
- Provides reproducible, biophysical readouts.
- Easy pipeline integration (straighforward assay development).
In-solution biophysics for challenging targets
- Suitable for dynamic, unstructured, or novel protein scaffolds.
- Measures interactions in solution (no surface artefacts).
- Includes stability, homogeneity, and structure information.
Marissa Baker
Genesis Molecular AI
Director of Biology
''Once we ran it on the FIDA, we actually quickly saw binding that correlated with our biochemical data and cellular data, and so we realized that in solution is actually more important than we realized''
Designing Proteins with Rapid AI–Lab Iterations: using FIDA to quantify in-solution protein behavior.
Watch a live conversation with Sharrol Bachas, PhD, CSO of Onava. Onava has developed a platform that continuously loops between computational design and wet-lab experimentation — using FIDA technology to generate the high-quality, quantitative data. A case study of how FIDA supports building AI-generated smart molecules for next-generation therapeutics.
Bridging design and discovery
FIDA speeds up de novo binder development by removing purification and assay adaptation steps. With FIDA binders can be screened and ranked (Kd) directly in lysate, using only microliter sample volumes. The FIDA data is absolute and quantitative, allowing fast, side-by-side comparison of designed variants under native conditions, which proves especially useful in de novo mini-binder protein screening.
Learn more during an exploratory call with Fidabio team.
We are happy to answer all of your questions. Book an exploratory call to learn more about FIDA and the match between your personal needs and what we can deliver. The call is non-binding and free of any charges, so feel free to fill the form!