Tozaro
How did Tozaro boost their screening throughput from 6 to up to 288 samples daily, reducing months of work to just days?
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Tozaro (Previously MIP Discovery / Diagnostics) ingeniously reimagined its affinity reagent screening workflow using FIDA, transforming interaction analysis from slow and laborintensive to fast and precise. The benefit? They managed to achieve greater efficiency, scalability, and decision-making power in molecular interaction analysis
Problem:
Traditional techniques such as Surface Plasmon Resonance (SPR) created throughput bottlenecks, limiting the ability to evaluate large polymer libraries quickly and cost-effectively.
Solution:
FIDA-Driven Workflow:
1. Primary Screen
Rapid elimination of non-binders using a fluorescently labelled indicator. Throughput: up to 3× 96-wellplates/day (288samples).
2. Confirmatory Screen
Quadruplicate measurements to validate interactions.
3. Kd Determination
Quantitative ranking via 8-point titration in 1.5–2 hours per sample.
4. Reverse Titration
Confirming specificity& binding stoichiometry.
After these steps, only selected candidates are progressed to SPR to assess surface immobilisation effects, eliminating the need for SPR in primary screening. By their innovative adaptation of FIDA, Tozaro replaced SPR for most polymer interaction screening analyses, streamlining research and accelerating discovery.
The benefits
By replacing SPR with FIDA for polymer interaction analysis, Tozaro streamlined research, reduced time-to-decision, and dramatically increased efficiency in polymer screening workflows.
SPR: 16+ days → FIDA: 30min - 3 hours
SPR: ~6 polymers/day → FIDA: up to 288 polymers/day
Ask yourself, what could you do with such time and sample savings?