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FIDA serves to measure molecular size in solution. It allows to evaluate both structure and function of the protein.
no buffer or concentration constraints.
What Does FIDA do?
Evaluates protein structure and function
Fida 1 evaluates both the structure and function of the protein or the particles of interest.
It measures the molecular interactions and properties of the construct of interest under native conditions, such as serum, plasma, cell lysate or fermentation media.
Provides an absolute size measurement (Rh)
Fida 1 directly measures the hydrodynamic radius of the molecule or construct of interest. With its unmatched sensitivity, it detects size changes of less than 5% in hydrodynamic radius. It has a dynamic size ranging from 0.5 to 500 nm Rh and can detect affinities from pM to mM.
Delivers 10 Essential Biophysical Parameters
FIDA Instruments deliver 10 parameters, including quality control . In one assay, FIDA generates more quantitative biophysical parameters than any other single instrument.
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What do you gain?
More time
Absolute measurements
Efficient sample use
Quality test samples in minutes.
“What we value about FIDA is the amount of information we can get from a tiny amount of protein in a single QC run. FIDA technology enables us to understand our proteins better - particularly when they behave in unexpected ways. We chose FIDA because it gives us that vital first result quickly - we don't waste our time optimising an assay that isn't going to work.”
Make you complex interactions simple.
“I like the ease of use and the straightforward data analysis and, that experiments can be performed in any type of buffers. We get data, where no other biophysical methods worked before."
In solution - native conditions
“FIDA is an in-solution technique, and thus an ideal binding assay for structurally diverse bsAbs because the analysis does not rely on potentially obstructive surface immobilization and hence it is able to perform characterizations that are unbiased by bsAb format and spatial orientation of the binding domains.”
It just works. Even with membrane proteins.
"After thorough comparisons, it was clear that the Fida 1, amongst others, presents unique opportunities in analysis of membrane proteins, which is essential to us."
