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A lipid transfer protein ensures nematode cuticular impermeability.
iScience 2022
Njume, F. N., Razzauti, A., Soler, M., Perschin, V., Fazeli, G., Bourez, A., Delporte, C., Ghogomu, S. M., Poelvoorde, P., Pichard, S., Birck, C., Poterszman, A., Souopgui, J., Van Antwerpen, P., Stigloher, C., Vanhamme, L., & Laurent, P.
This study identified GMAP-1, a secreted lipid transfer protein, as essential for maintaining the impermeability of the nematode cuticle in Caenorhabditis elegans, shuttling lipids to seal the cuticular barrier. To confirm GMAP-1 directly binds lipid, the authors used Flow Induced Dispersion Analysis (FIDA) to measure the interaction of fluorescently labeled phosphatidylcholine (NBD-PC) with the purified protein, determining a dissociation constant (Kd) of ~3 µM and resolving the size of the free lipid versus the lipid–protein complex in solution. It is an example of using FIDA to measure protein–lipid binding affinity in solution.
