Let us send you the file to your email.
If you wish, you can choose to receive more literature on the topic by agreeing to receive other communication.
You will receive the file on your email.
Bi-directional protein-protein interactions control liquid-liquid phase separation of PSD-95 and its interaction partners
iScience 2022
Christensen, N. R., Pedersen, C. P., Sereikaite, V., Pedersen, J. N., Vistrup-Parry, M., Sørensen, A. T., Otzen, D., Teilum, K., Madsen, K. L., & Strømgaard, K.
This study mapped how protein–protein interactions drive liquid–liquid phase separation (LLPS) of PSD-95, the master scaffold of the neuronal postsynaptic density, identifying the minimal Stargazin (TARP-γ2) peptide that induces LLPS and showing that PSD-95 inhibitors can also promote it. Flow Induced Dispersion Analysis (FIDA) was used in a supporting role to measure the hydrodynamic radius of PSD-95 with and without Stargazin peptides, with complexes appearing larger than PSD-95 alone. It is an example of using FIDA to measure protein complex size in solution.
