Researchers used computational design (PROSS2) to engineer heat-stable variants of the Chagas disease antigen Tc24, raising its melting temperature from 53.5 °C in the wild-type to 73.6 °C in the top variant while boosting yield and extending solution stability from 12 to 70 days. Flow Induced Dispersion Analysis (FIDA), on a Fida 1 instrument, was used to measure the hydrodynamic radius of the Tc24 variants across repeated freeze-thaw cycles and after lyophilization, confirming they stayed monodisperse and structurally unchanged. The most stable variant retained antigenicity in patient sera and was adapted into a lateral flow test prototype. It is an example of using FIDA to assess protein sample integrity and stability under real-world storage and handling stresses.