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FibrilPaint to determine the length of Tau amyloids in fluids
PNAS 2025
Júlia Aragonès Pedrola, Françoise A. Dekker, Tommaso Garfagnini, Guy Mayer, Margreet B. Koopman, Menno Bergmeijer, Friedrich Förster, Jeroen J. M. Hoozemans, Henrik Jensen, Assaf Friedler, Stefan G. D. Rüdiger
This study introduces FibrilPaint1, a fluorescent peptide that binds Tau amyloid fibrils, and pairs it with Flow Induced Dispersion Analysis (FIDA) to determine Tau fibril length directly in solution. On a Fida 1 instrument, FIDA measured the hydrodynamic radius (Rh) of the FibrilPaint–fibril complexes, turning the readout into a "molecular ruler" that sizes fibrils across a wide range, tracks their growth during aggregation, and detects them at picomolar concentrations in human serum and in patient-derived Alzheimer's, frontotemporal-dementia and corticobasal-degeneration samples. It is an example of using FIDA to size amyloid fibrils and their complexes in biofluids.
