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High Throughput detergent screening for membrane proteins using nanoliters of sample
Isabel Moraes, PhD; Amy Danson PhD; Riccardo Marabini, PhD
This poster, led by the National Physical Laboratory (NPL) with Fida Biosystems, demonstrates how Flow Induced Dispersion Analysis (FIDA), on a Fida 1 instrument, was used to screen 11 detergents for their ability to solubilise a GFP-tagged membrane protein (GFP-LacY) directly in crude cell lysate, measuring solubilisation efficiency and polydispersity (PDI) from just 1.5 µL of sample. The FIDA-based detergent ranking matched fluorescence size-exclusion chromatography (FSEC) while cutting screening from ~20 hours to under 3 and sample volume nearly 1,000-fold. It is an example of using FIDA to rapidly screen and characterise membrane protein samples ahead of structural biology work.
