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Mechanistic insights into substrate recognition by Pseudouridine Synthases
Yasmin Stone, Xiaomeng Zhang, Ella Maunder, Ziyi Qian, and Ting-Yu Lin
This poster from Durham University demonstrates how Flow Induced Dispersion Analysis (FIDA), on a Fida Neo instrument, was used to determine the binding affinity of pseudouridine synthase (PUS) enzymes to their tRNA substrates. FIDA quantified PUS–tRNA binding in solution, complementing cryo-EM structures and primer-extension assays that revealed position-specific substrate recognition. It is an example of using FIDA to characterise protein–RNA binding affinity for enzyme–substrate recognition studies.
