Let us send you the file to your email.
If you wish, you can choose to receive more literature on the topic by agreeing to receive other communication.
You will receive the file on your email.
Protein Quantification in plasma (probing immune responses in patients)
Nicklas N. Poulsen, PhD; Morten E. Pedersen, PhD; Jesper Østergaard, PhD;, Nickolaj J. Petersen; PhD; Niels H. H.Heegaard, PhD; and Henrik Jensen, PhD
This poster demonstrates how Flow Induced Dispersion Analysis (FIDA) was used to quantify human serum albumin in blood plasma and to detect anti-DNA autoantibodies in plasma from patients with systemic lupus erythematosus (SLE). By tracking changes in the apparent size of a fluorescent protein or DNA indicator on binding, FIDA measured plasma protein concentration with under 7% standard deviation and distinguished patient-specific autoantibody binding to different DNA sequences. It is an example of using FIDA to quantify proteins and characterise antibody binding directly in complex biological matrices such as human plasma.
