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Virus-modified paraspeckle-like condensates are hubs for viral RNA processing and their formation drives genomic instability
Nature Communications 2024
Harper, K.L., Harrington, E.M., Hayward, C. et al.
This study shows that Kaposi's sarcoma-associated herpesvirus (KSHV) remodels host paraspeckles into virus-modified paraspeckle-like condensates that act as hubs for viral RNA processing and drive host genomic instability, with the viral protein ORF11 identified as a scaffold that drives their assembly by liquid-liquid phase separation. Flow Induced Dispersion Analysis (FIDA) was used to confirm this phase-separation behavior: purified recombinant ORF11 flowed through a capillary produced characteristic fluorescent signal spikes as droplets formed, giving an orthogonal, in-solution confirmation alongside microscopy. It is an example of using FIDA to detect protein liquid-liquid phase separation in solution.
