Fida Evolve

FIDA (Flow-Induced Dispersion Analysis) can be applied to a wide range of sample types because it measures molecules directly in solution, without requiring immobilization or separation. This makes it particularly well suited for working with complex and native systems.

FIDA is especially valuable for analyzing complex, native or unpurified samples. It can measure targets directly in matrices such as serum, plasma, cerebrospinal fluid, cell lysates, or fermentation media. This allows experiments to be performed in environments that are closer to native conditions, avoiding purification steps that may alter molecular behavior or introduce bias.

It also performs well with heterogeneous or partially purified samples. By measuring absolute molecular size (hydrodynamic radius), FIDA can distinguish between different populations in solution, such as bound and unbound species or different oligomeric states. This makes it useful for assessing sample quality, polydispersity, and interaction specificity in real-world samples.

In addition, FIDA is robust across a wide range of buffer and formulation conditions, including high salt, detergents, and crowding agents. Combined with its low sample consumption, this supports applications such as formulation screening, stability studies, and work with limited or difficult-to-obtain material. For purified samples, FIDA is commonly used with proteins, peptides, antibodies, small molecules, and nucleic acids. In these well-defined systems, it provides precise and quantitative readouts such as hydrodynamic radius, binding affinity, and interaction profiles, enabling detailed characterization of molecular interactions.

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Autosampler temperature control: 5°- 95°C (41°-203°F)
Capillary temperature control: 15°- 55°C (59°-131°F)

Kinetic processes from seconds to hours.

The system measures dissociation constants (Kᴅ) from picomolar (pM) to millimolar (mM).

• Click-in Detectors and Capillaries: No training, tools, calibration nor downtime, plus reduced run-to-run variability. In the Evolve model we redefined the usability by optimizing the manual handling. This model features click-on detectors and capillaries, which speed up the assays and dimmish any chance of manual handling errors. Every interaction with this instrument, from inserting a capillary to swapping a detector, is designed for clarity and confidence.

• Fast Calibration-Free Pressure Control: Fida Evolve features a fast pre-calibrated, self-regulating pressure control, making FIDA assays even easier.
• Compact, Integrated Design: The detector is now fully integrated into the main unit, resulting in: a smaller overall footprint easier installation in space-limited labs.
• Expanded Temperature Control: autosampler temperature control: 5°- 95°C (41°-203°F); capillary temperature control: 15°- 55°C (59°-131°F)
• Pause and edit functions: allow for Flexible Mid-Run Editing during a live experiment. Now you can just pause a sequence during your exploratory and adaptive workflows.

Fida Evolve introduces an extra Kd determination method, which is optimized for high-throughput workflows and early-stage screening. It can be used before running a full titration.

Instead of measurements at discrete concentrations, a Taylor-dispersion–induced concentration gradient inside the capillary exposes the target to a continuous titration of analytes, from which the binding affinity is derived. The result is a fast, low-resource method that retains in-solution accuracy and requires no surface immobilization, making it ideal for prioritising hits without compromising data quality.
Read more in this publication. Or watch this webinar.

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FIDA delivers 3 simultaneous measurements – for increased precision, reproducibility and extended workflow capacities.
Absolute size (hydrodynamic radius) measurement provides a direct, calibration-free assessment of molecular size in solution. By tracking hydrodynamic radius, FIDA reveals size changes due to binding, oligomerization, or aggregation. Because the readout is absolute, it offers a high level of confidence when monitoring subtle structural differences, conformational states, or purity. It is especially powerful in the era of AI, where absolute data is necessary for training analytical models.
Lambda Dynamics (Ratiometric fluorescence detection) readout: by capturing shifts in the emission profile of the fluorescent tracer, it enables specific and quantitative analysis of interactions that would otherwise go unnoticed. This makes it ideal for fragment screening, small molecule profiling, and other applications where size-based detection is limited.
Binding Related Intensity Change readout tracks changes in fluorescence intensity that occur upon binding. This direct signal allows for sensitive detection of binding events, including those with low affinity or occurring in complex or crude samples. It offers fast confirmation of interaction presence and can guide assay design or screening decisions.