Quantification of in-solution sample aggregation is performed ubiquitously, all throughout Fida 1 measurement. Sample molecules that form large aggregates (big soluble/insoluble particles) often cause high light scattering and are detected as distinct spikes in the raw FIDA signal. As shown in figure 2A (in green), large aggregates appear as signal spikes on raw Fida 1 data. These sample aggregates can be discretely quantified using the ‘spike counter tool’ integrated in FIDA analysis software.
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