FIDA requires a fluorescent signal coming from your sample that can be detected by one of our detectors. It can either be emitting from a label or from the molecule itself, i.e. label-free. The signal coming from your sample that can be detected by one of our detectors: 480, 550, 640, 280.
FIDA uses a LED excitation and amplifies the emission signal by PMT. Therefore, FIDA does not use any laser to get a signal, and the sample is not heated. Note that FIDA delivers two orthogonal readouts: absolute size (hydrodynamic radius) and fluorescence.