How does Fida Instrument detect a signal?

FIDA requires a fluorescent signal coming from your sample that can be detected by one of our detectors. It can either be emitting from a label or from the molecule itself, i.e. label-free. The signal coming from your sample that can be detected by one of our detectors: 480, 550, 640, 280. FIDA uses a LED excitation and amplifies the emission signal by PMT. Therefore, FIDA does not use any laser to get a signal, and the sample is not heated. Note that FIDA delivers three orthogonal readouts: absolute size (hydrodynamic radius), fluorescence measurement (Binding Related Intensity Change - BRIC) and Fida Lambda Dynamics, which refers to the measurement of the shifts in the wavelength (lambda) of the detected fluorescence signal, which occurs when binding events change the environment of the fluorophore or other optical markers.

Read more about:
Fida Lambda Dynamics https://www.fidabio.com/blogs/enhanced-fida-analysis-of-small-molecule-protein-interactions
BRIC: https://www.fidabio.com/blogs/understanding-bric-in-fida-technology-a-comprehensive-overview

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