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Protein analysis and characterisation
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What is FIDA?

FIDA stands for Flow Induced Dispersion Analysis. It is a first-principle based, in-solution technology, which provides a precise, quantitative, and highly robust method to determine how changes to an analyte affect the analyte. The changes that may be assessed are numerous ranging from adding a binding partner to changes in the environments including ionic strength, pH, buffers and different crude matrixes, temperature etc. The typical readout is hydrodynamic radi which provides information about quantity, affinity, sample integrity, changes in oligomeric state, aggregation, stoichiometry etc.

FIDA is based on first principles of physics and fluid mechanics of a laminar flow and Taylor dispersion. FIDA technology takes advantage of these two principles by measuring fluorescence of particles in the laminar flow and analysing their dispersion over time. Following signal analysis, gives rise to an accurate assessment of molecular diffusivity and hydrodynamic radius. For instance, the change in the apparent hydrodynamic radius of a binder in the presence of its ligand reflects the strength of the interaction.