The smallest molecule that can be measured by Fida 1 can be down to 0.5 nm in hydrodynamic radius (Rh). This could be a species like small biomolecules or peptides. The largest molecules that can be measured by Fida 1 can be species of up to 500 nm in Rh. This includes large nanoparticles (like LNPs, OMVs, etc.), liposomes, exosomes or virus-like particles.